NARFL-Specific Polyclonal antibody

NARFL-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20209-1-AP

Synonyms

CIAO3, HPRN, IOP1, LET1L, NARFL, NARFL-Specific, PRN, Protein related to Narf

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Validation Data Gallery

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  WB analysis of HEK-293 using 20209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 20209-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20209-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20209-1-AP (NARFL-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 20209-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20209-1-AP (NARFL-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20209-1-AP targets NARFL-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear prelamin A recognition factor-like
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: NM_022493
• Gene Symbol: NARFL
• Gene ID (NCBI): 64428
• RRID: AB_10694435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. [18270200], [16956324] The antibody is specific to NARFL.

Publications

Species	Application	Title
human	WB	Free Radic Biol Med; The iron-sulfur cluster assembly network component NARFL Is a key element in the cellular defense against oxidative stress.; Authors - Monique Corbin