NARG1 Polyclonal antibody

NARG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 23845-1-AP

Synonyms

NMDA receptor-regulated protein 1, N-alpha-acetyltransferase 15, NatA auxiliary subunit, NAA15, N terminal acetyltransferase, Gastric cancer antigen Ga19

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Validation Data Gallery

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  WB analysis of HeLa using 23845-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23845-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 23845-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 23845-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 23845-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23845-1-AP (NARG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23845-1-AP targets NARG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NARG1 fusion protein Ag20842
• Full Name: NMDA receptor regulated 1
• Calculated Molecular Weight: 866 aa, 101 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC093928
• Gene Symbol: NARG1
• Gene ID (NCBI): 80155
• RRID: AB_2879338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAA15 (N-alpha-acetyltransferase 15) is a subunit of both the NatA and NatE complexes, and its role is to position the catalytic subunits in close vicinity to the nascent polypeptides (PMID: 33557580). Human NAA15 encodes an 866 amino acid (~105 kDa) protein, NAA15, containing tetratricopeptide repeat domains and a putative bipartite nuclear localization signal (PMID: 29656860). NAA15 was implicated in human disease and loss-of-function NAA15 variants in large cohorts of patients with autism spectrum disorder and/or ID (PMID: 33103328).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zehua (Verified Customer) (07-09-2018)

Samples from ProteinTech. Works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7