Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23845-1-AP targets NARG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20842 Product name: Recombinant human NARG1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-350 aa of BC093928 Sequence: MPAVSLPPKENALFKRILRCYEHKQYRNGLKFCKQILSNPKFAEHGETLAMKGLTLNCLGKKEEAYELVRRGLRNDLKSHVCWHVYGLLQRSDKKYDEAIKCYRNALKWDKDNLQILRDLSLLQIQMRDLEGYRETRYQLLQLRPAQRASWIGYAIAYHLLEDYEMAAKILEEFRKTQQTSPDKVDYEYSELLLYQNQVLREAGLYREALEHLCTYEKQICDKLAVEETKGELLLQLCRLEDAADVYRGLQERNPENWAYYKGLEKALKPANMLERLKIYEEAWTKYPRGLVPRRLPLNFLSGEKFKECLDKFLRMNFSKGCPPVFNTLRSLYKDKEKVAIIEELVVGYE Predict reactive species |
| Full Name | NMDA receptor regulated 1 |
| Calculated Molecular Weight | 866 aa, 101 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC093928 |
| Gene Symbol | NARG1 |
| Gene ID (NCBI) | 80155 |
| RRID | AB_2879338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9BXJ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NAA15 (N-alpha-acetyltransferase 15) is a subunit of both the NatA and NatE complexes, and its role is to position the catalytic subunits in close vicinity to the nascent polypeptides (PMID: 33557580). Human NAA15 encodes an 866 amino acid (~105 kDa) protein, NAA15, containing tetratricopeptide repeat domains and a putative bipartite nuclear localization signal (PMID: 29656860). NAA15 was implicated in human disease and loss-of-function NAA15 variants in large cohorts of patients with autism spectrum disorder and/or ID (PMID: 33103328).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NARG1 antibody 23845-1-AP | Download protocol |
| IHC protocol for NARG1 antibody 23845-1-AP | Download protocol |
| WB protocol for NARG1 antibody 23845-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
All three reviews are for Western Blot applications at a 1:1000 dilution. Users report the antibody works well with overnight cold room incubation, producing a clean signal at the expected molecular weight. Tested on HCT116 and MCF-7 cell lines, the antibody consistently delivers reliable results. Average rating is approximately 4.7 out of 5, with two reviewers giving 5 stars and one giving 4 stars. Overall, customers express satisfaction with the product's performance and specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nathalie (Verified Customer) (03-05-2026) | Works well (overnight cold room incubation)
|
Morgane (Verified Customer) (10-01-2025) | Migrates at expected size and clean signal
 |
Zehua (Verified Customer) (07-09-2018) | Samples from ProteinTech. Works very well.
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