NAT10 Polyclonal antibody

• Featured Product
• KD/KO Validated

NAT10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

55

Reviews (3)

Cat no : 13365-1-AP

Synonyms

N-acetyltransferase 10, N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP

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Validation Data Gallery

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  WB analysis of HeLa using 13365-1-AP

  WB result of NAT10 antibody (13365-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells.

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  WB analysis using 13365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13365-1-AP

  WB result of NAT10 antibody (13365-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells.

• 

  IP experiment of mouse testis using 13365-1-AP

  IP result of anti-NAT10 (IP:13365-1-AP, 4ug; Detection:13365-1-AP 1:8000) with mouse testis tissue lysate 1120 ug.

• 

  IHC staining of human ovary cancer using 13365-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13365-1-AP (NAT10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 44 publications below
• IHC: See 8 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 5 publications below

Product Information

13365-1-AP targets NAT10 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAT10 fusion protein Ag4184
• Full Name: N-acetyltransferase 10 (GCN5-related)
• Calculated Molecular Weight: 1025 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC035558
• Gene Symbol: NAT10
• Gene ID (NCBI): 55226
• RRID: AB_2148944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA. (24786082)

Publications

Species	Application	Title
human	WB,IP	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango ; KO Validated
human	WB, IF	Science; Chemical inhibition of NAT10 corrects defects of laminopathic cells.; Authors - Delphine Larrieu ; KD Validated
human	WB	Cell Host Microbe; Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.; Authors - Kevin Tsai ; KO Validated
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
mouse	WB	Nat Commun; Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.; Authors - Gabriel Balmus ; KO Validated
mouse	WB,IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SONAM (Verified Customer) (12-18-2023)

Antibody works really well. No Nonspecific bindings.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cellls

FH

Mattia (Verified Customer) (09-12-2019)

Clear band at the right size, when antibody used at 1:1500 dilution in 5% milk an incubated at 2hrs at 25C. Band is a bit streaky, may require further optimisation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: HeLa, HEK293T

FH

Jonathan (Verified Customer) (11-29-2018)

• Applications: Western Blot, Immunofluorescence,

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