NBR1 Monoclonal antibody

NBR1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

Unconjugated

CloneNo.

1F2B2

Cat no : 68062-1-Ig

Synonyms

1A1 3B, 1A13B, FLJ55359, FLJ98272, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B



Tested Applications

Positive WB detected inNCCIT cells, hTERT-RPE1 cells, HeLa cells, HEK-293 cells, K-562 cells, rat testis tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

68062-1-Ig targets NBR1 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NBR1 fusion protein Ag8652
Full Name neighbor of BRCA1 gene 1
Calculated molecular weight 966 aa, 107 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC009808
Gene symbol NBR1
Gene ID (NCBI) 4077
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).

Protocols

Product Specific Protocols
WB protocol for NBR1 antibody 68062-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols