CoraLite®594 Anti-Mouse NCAM1/CD56 Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow cells using CL594-98118

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite®594 Anti-Mouse NCAM1/CD56 Rabbit RecAb (CL594-98118, Clone:240936A4)(red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL594-98118

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite®594 Anti-Mouse NCAM1/CD56 Rabbit RecAb (CL594-98118, Clone:240936A4) (red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were not fixed.

NCAM1/CD56 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-98118

Clone No.240936A4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD56, Ncam1, 240936A4, E NCAM, Ncam

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98118 targets NCAM1/CD56 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0652

  Product name: Recombinant Mouse NCAM-1/CD56 protein (His Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 20-711 aa of

  Sequence: LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKTLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEFQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEESWHFKWYDAKEANMEGIVTIMGLKPETRYSVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST

• Full Name: neural cell adhesion molecule 1
• Calculated Molecular Weight: 119 kDa
• Gene Symbol: Ncam1
• Gene ID (NCBI): 17967
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13595-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56, NCAM, and MSK39) is a membrane-bound cell surface glycoprotein and a member of the immunoglobulin supergene family, which induces cell-to-cell interactions during embryonic development, cell migration, and organogenesis (PMID: 8013635; 1855476; 1391354). Mouse natural killer (NK) cells do not express the murine homolog of CD56 (PMID: 17100875). The molecular weight of NCAM1 changes from the 120 kDa isoform in normal tissue to the 140/180 kDa isoforms in tumors (PMID: 10086383).