Tested Applications
| Positive WB detected in | HeLa cells, PC-12 cells, J774A.1 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33595-1-AP targets NCAPH2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38706 Product name: Recombinant mouse Ncaph2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-607 aa of NM_001115132 Sequence: VMPEEAADLDAEAMPESLRYEELVRRNVELFIATSQKFIQETELSQRIRDWEDTIQPLLQEQEQHVPFDIHIYGDQLASRFPQLNEWCPFSELVAGQPAFEVCRSMLASLQLANDYTVEITQQPGLEAAVDTMSLRLLTHQRAHTRFQTYAAPSMAQP* Predict reactive species |
| Full Name | non-SMC condensin II complex, subunit H2 |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 68-80 kDa |
| GenBank Accession Number | NM_001115132 |
| Gene Symbol | Ncaph2 |
| Gene ID (NCBI) | 52683 |
| RRID | AB_3743023 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8BSP2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NCAPH2 is a subunit of the condensin-2 complex involved in chromosome condensation during mitosis and meiosis. Peripheral blood DNA methylation levels in the NCAPH2/LMF2 promoter region were significantly decreased in patients with AD and those with amnestic mild cognitive impairment (aMCI); therefore, these are considered to be a potentially useful biomarker for the diagnosis of AD. (PMID: 33603659)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NCAPH2 antibody 33595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
