Tested Applications
| Positive WB detected in | U-937 cells, Raji cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28187-1-AP targets NCF1/p47 phox in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28089 Product name: Recombinant human NCF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-190 aa of BC002816 Sequence: KWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSE Predict reactive species |
| Full Name | neutrophil cytosolic factor 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002816 |
| Gene Symbol | p47phox/NCF1 |
| Gene ID (NCBI) | 653361 |
| RRID | AB_2881084 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14598 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NCF1/p47 phox antibody 28187-1-AP | Download protocol |
| WB protocol for NCF1/p47 phox antibody 28187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NCF1/p47 phox Polyclonal antibody (Cat# 28187-1-AP) has 13 citations. These appear in journals including Advanced Materials, Journal of Hazardous Materials, Free Radical Biology and Medicine, Clinical Nutrition, Oxidative Medicine and Cellular Longevity, Frontiers in Immunology, Journal of Ethnopharmacology, Drug Design Development and Therapy, Journal of Physiology and Biochemistry, Laboratory Investigation, BMC Pulmonary Medicine, Biochemical and Biophysical Research Communications, and Journal of Healthcare Engineering. Research fields span oxidative stress, inflammation, fibrosis, liver disease, cardiovascular pathology, oncology, and pulmonary medicine.
| Species | Application | Title |
|---|---|---|
J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. | ||
Free Radic Biol Med Macrophage NADPH oxidase inhibition by ultralow-dose dextromethorphan alleviates DSS-induced inflammation and colitis | ||
Clin Nutr Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress. | ||
Oxid Med Cell Longev Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. | ||
Front Immunol Combined single-cell RNA-seq and bulk RNA-seq construction of M2 TAMs signature for predicting HNSCC prognosis and immunotherapy. |
