NCKAP1 Polyclonal antibody, PBS Only

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• NCKAP1 Polyclonal antibody, PBS Only (12140-1-PBS)

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  WB analysis of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  WB analysis using 12140-1-AP (same clone as 12140-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12140-1-AP (NCKAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody (12140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 12140-1-AP (same clone as 12140-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using NCKAP1 antibody (12140-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

NCKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 12140-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

HEM2, KIAA0587, Membrane-associated protein HEM-2, NAP 1, NAP1

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Product Information

12140-1-PBS targets NCKAP1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2788

  Product name: Recombinant human NAP1;NCKAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 779-1129 aa of BC015025

  Sequence: HGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

• Full Name: NCK-associated protein 1
• Calculated Molecular Weight: 129 kDa
• Observed Molecular Weight: 115-125 kDa
• GenBank Accession Number: BC015025
• Gene Symbol: NCKAP1
• Gene ID (NCBI): 10787
• RRID: AB_10666284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2A7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.