NCOR2 Polyclonal antibody, PBS Only

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• NCOR2 Polyclonal antibody, PBS Only (29952-1-PBS)

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  WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

  MOLT-4 cells were subjected to Tris-Acetate gel system followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29952-1-AP (same clone as 29952-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NCOR2 antibody (29952-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

NCOR2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29952-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

29952-1-PBS targets NCOR2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30727

  Product name: Recombinant human NCOR2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1165-1363 aa of NM_006312

  Sequence: SGVKQEQLSPRGQAGPPESLGVPTAQEASVLRGTALGSVPGGSITKGIPSTRVPSDSAITYRGSITHGTPADVLYKGTITRIIGEDSPSRLDRGREDSLPKGHVIYEGKKGHVLSYEGGMSVTQCSKEDGRSSSGPPHETAAPKRTYDMMEGRVGRAISSASIEGLMGRAIPPERHSPHHLKEQHHIRGSITQGIPRSY

• Full Name: nuclear receptor co-repressor 2
• Calculated Molecular Weight: 274 kDa
• Observed Molecular Weight: 274 kDa
• GenBank Accession Number: NM_006312
• Gene Symbol: NCOR2
• Gene ID (NCBI): 9612
• RRID: AB_3086197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y618
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).