NCS1 Monoclonal antibody

NCS1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67616-1-Ig
Clone No.2F10C8

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

DKFZp761L1223, FLUP, FREQ, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein, NCS 1, NCS1, Neuronal calcium sensor 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inU-251 cells, U-87 MG cells, pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67616-1-Ig targets NCS1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29808

Product name: Recombinant human FREQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-190 aa of BC004856

Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

Predict reactive species
Full Name frequenin homolog (Drosophila)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC004856
Gene Symbol NCS1
Gene ID (NCBI) 23413
RRIDAB_2882819
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NCS1 antibody 67616-1-IgDownload protocol
IHC protocol for NCS1 antibody 67616-1-IgDownload protocol
IF protocol for NCS1 antibody 67616-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Int J Mol Sci

Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons

Authors - Elena G Varlamova
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