NCS1 Monoclonal antibody

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• NCS1 Monoclonal antibody (67616-1-Ig)

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NCS1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67616-1-Ig Copied!

Clone No. 2F10C8

Publications (1)

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Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA
For FC, select conjugated version.

Synonyms

FREQ, FLUP, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein

• 

  WB analysis of U-87 MG using 67616-1-Ig

  WB result of NCS1 antibody (67616-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCS1 transfected U-87 MG cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67616-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67616-1-Ig (NCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 67616-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 67616-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 67616-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCS1 antibody (67616-1-Ig, Clone: 2F10C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 67616-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 67616-1-Ig against Human FREQ were performed. The affinity constant is below 1 pM. against Human RBM8A were performed. The affinity constant is below 1 pM. against Human RBM8A were performed. The affinity constant is 0.119 nM. against Human PGAM1 were performed. The affinity constant is 18.6 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: U-251 cells, U-87 MG cells, pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67616-1-Ig targets NCS1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29808

  Product name: Recombinant human FREQ protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-190 aa of BC004856

  Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

• Full Name: frequenin homolog (Drosophila)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC004856
• Gene Symbol: NCS1
• Gene ID (NCBI): 23413
• RRID: AB_2882819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62166
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-NCS-1 (Neuronal Calcium Sensor-1) antibody, catalog number 67616-1-Ig, has a total of 1 citation. It appears in the International Journal of Molecular Sciences (IF 5.6), published in December 2022. The study investigated how NCS-1 protects cortical neurons from hyperexcitation and calcium overload during ischemia by preserving GABAergic neurons. The research field is neuroscience, specifically neuroprotection and cerebral ischemia. The application used was immunofluorescence in mouse tissue.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Int J Mol Sci; Neuronal Calcium Sensor-1 Protects Cortical Neurons from Hyperexcitation and Ca2+ Overload during Ischemia by Protecting the Population of GABAergic Neurons; Authors - Elena G Varlamova