NCS1 Monoclonal antibody, PBS Only (Capture)

• 

  WB analysis of U-87 MG using 67616-1-Ig (same clone as 67616-1-PBS)

  WB result of NCS1 antibody (67616-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCS1 transfected U-87 MG cells. This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67616-1-Ig (same clone as 67616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67616-1-Ig (NCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCS1 antibody (67616-1-Ig, Clone: 2F10C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51282-1

  Cytometric bead array standard curve of MP51282-1, FREQ Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67616-1-PBS. Detection antibody: 67616-2-PBS. Standard:Ag29808. Range: 1.563-100 ng/mL.

• Featured Product
• KD/KO Validated

NCS1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 67616-1-PBS

Clone No.2F10C8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

FREQ, FLUP, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein

---

Product Information

67616-1-PBS targets NCS1 as part of a matched antibody pair:

MP51282-1: 67616-1-PBS capture and 67616-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29808

  Product name: Recombinant human FREQ protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-190 aa of BC004856

  Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

• Full Name: frequenin homolog (Drosophila)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC004856
• Gene Symbol: NCS1
• Gene ID (NCBI): 23413
• RRID: AB_2882819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62166
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.