NCS1 Recombinant monoclonal antibody

• 

  WB analysis using 87673-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87673-1-RR (NCS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

NCS1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87673-1-RR

Clone No.253130F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FREQ, FLUP, Frequenin homolog, frequenin homolog (Drosophila), Frequenin like protein

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Tested Applications

• Positive WB detected in: A431 cells, U-87 MG cells, SH-SY5Y cells, PC-3 cells, mouse brain tissue, rat brain tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87673-1-RR targets NCS1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5439

  Product name: Recombinant human NCS1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 1-190 aa of NM_014286.3

  Sequence: MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV

• Full Name: frequenin homolog (Drosophila)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: NM_014286.3
• Gene Symbol: NCS1
• Gene ID (NCBI): 23413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62166-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal Calcium Sensor 1 is a member of the NCS protein family, a group of evolutionarily conserved, calcium-binding proteins predominantly expressed in the nervous system and neuroendocrine cells. NCS-1 is the vertebrate homolog of the extensively studied Drosophila protein Frequenin. It plays a crucial and multifaceted role in regulating neuronal signaling, synaptic plasticity, and neurotransmitter release.