Validation Data Gallery
|Positive WB detected in
|HeLa cells, A2780 cells, human spleen tissue, Jurkat cells, mouse testis tissue
|Positive IP detected in
|Positive IHC detected in
|human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
18932-1-AP targets NDC80 in WB, IP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
|human, rat, mouse
|Host / Isotype
|Rabbit / IgG
|NDC80 fusion protein Ag8065
|NDC80 homolog, kinetochore complex component (S. cerevisiae)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
J Cell Biol
PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size
Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.
Upregulation of nuclear division cycle 80 contributes to therapeutic resistance via the promotion of autophagy-related protein-7-dependent autophagy in lung cancer
Onco Targets Ther
Targeting DTL induces cell cycle arrest and senescence and suppresses cell growth and colony formation through TPX2 inhibition in human hepatocellular carcinoma cells.
NDC80 status pinpoints mitotic kinase inhibitors as emerging therapeutic options in clear cell renal cell carcinoma