NDC80 Polyclonal antibody

NDC80 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18932-1-AP

Synonyms

HEC, HEC1, HsHec1, hsNDC80, Kinetochore protein Hec1, KNTC2, NDC80, TID3



Tested Applications

Positive WB detected inHeLa cells, A2780 cells, human spleen tissue, Jurkat cells, mouse testis tissue
Positive IP detected inA2780 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18932-1-AP targets NDC80 in WB, IP, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDC80 fusion protein Ag8065
Full Name NDC80 homolog, kinetochore complex component (S. cerevisiae)
Calculated molecular weight 74 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC035617
Gene symbol NDC80
Gene ID (NCBI) 10403
RRIDAB_2149750
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDC80 antibody 18932-1-APDownload protocol
IHC protocol for NDC80 antibody 18932-1-APDownload protocol
IP protocol for NDC80 antibody 18932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

J Cell Biol

PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size

Authors - Tomoaki Sobajima
humanWB

Oncotarget

Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.

Authors - Yanwei Liu
humanWB,IHC

Front Pharmacol

Upregulation of nuclear division cycle 80 contributes to therapeutic resistance via the promotion of autophagy-related protein-7-dependent autophagy in lung cancer

Authors - Xi Chen
  • KD Validated
humanWB

Onco Targets Ther

Targeting DTL induces cell cycle arrest and senescence and suppresses cell growth and colony formation through TPX2 inhibition in human hepatocellular carcinoma cells.

Authors - Yu-Chia Chen
IHC

iScience

NDC80 status pinpoints mitotic kinase inhibitors as emerging therapeutic options in clear cell renal cell carcinoma

Authors - Cheng Hu