HEC1/HEC/NDC80 Polyclonal antibody

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• HEC1/HEC/NDC80 Polyclonal antibody (18932-1-AP)

HEC1/HEC/NDC80 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18932-1-AP Copied!

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NDC80, HEC, HEC1, HsHec1, hsNDC80

• 

  WB analysis using 18932-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human spleen using 18932-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of A2780 using 18932-1-AP

  IP result of anti-NDC80 (IP:18932-1-AP, 4ug; Detection:18932-1-AP 1:500) with A2780 cells lysate 960ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 18932-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NDC80 antibody (18932-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A2780 cells, human spleen tissue, Jurkat cells, mouse testis tissue
• Positive IP detected in: A2780 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18932-1-AP targets HEC1/HEC/NDC80 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8065

  Product name: Recombinant human NDC80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 290-642 aa of BC035617

  Sequence: PNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

• Full Name: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC035617
• Gene Symbol: NDC80
• Gene ID (NCBI): 10403
• RRID: AB_2149750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14777
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The NDC80 antibody (Cat# 18932-1-AP) has 9 total citations published in journals including J Transl Med, CNS Neurosci Ther, Oncotarget, J Cell Biol, Front Pharmacol, Cell Signal, iScience, Onco Targets Ther, and Discov Oncol. Research fields span cancer biology (glioblastoma, lung cancer, colorectal cancer, renal cell carcinoma, hepatocellular carcinoma, bladder cancer) and mitotic spindle biology, with applications primarily in WB, IF, IHC, and IP.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Transl Med; Disruption of the NDC80-Nek2 axis suppresses glioblastoma stemness and enhances therapeutic efficacy.; Authors - Rui Niu ; KD Validated
human	WB,IF	CNS Neurosci Ther; NDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment; Authors - Weijie Ye ; KD Validated
human	WB	Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human	IP	J Cell Biol; PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size; Authors - Tomoaki Sobajima
human	WB,IHC	Front Pharmacol; Upregulation of nuclear division cycle 80 contributes to therapeutic resistance via the promotion of autophagy-related protein-7-dependent autophagy in lung cancer; Authors - Xi Chen ; KD Validated
	WB	Cell Signal; LOH12CR2 activated by sodium butyrate suppresses tumorigenesis in colorectal cancer via METTL14-mediated N6-methyladenosine modification of SPC24 mRNA; Authors - Yanbing Lin