NDRG1 Recombinant antibody

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  WB analysis using 85141-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85141-4-RR (NDRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 85141-4-RR

  IP result of anti-NDRG1 (IP:85141-4-RR, 4ug; Detection:85141-4-RR 1:1000) with HeLa cells lysate 1520 ug.

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  IHC staining of human lung cancer using 85141-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 85141-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85141-4-RR (NDRG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 85141-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug NDRG1 Recombinant antibody (85141-4-RR, Clone:242322F7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 85141-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85141-4-RR against Human NDRG1 were performed. The affinity constant is below 1 pM.

NDRG1 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 85141-4-RR

Clone No.242322F7

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

242322F7, CMT4D, Differentiation-related gene 1 protein, DRG1, DRG-1

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, rat kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85141-4-RR targets NDRG1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NDRG1 fusion protein Ag25424
• Full Name: N-myc downstream regulated 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 45-46 kDa
• GenBank Accession Number: BC006260
• Gene Symbol: NDRG1
• Gene ID (NCBI): 10397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.