Tested Applications
| Positive WB detected in | human heart tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rat heart tissue |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67191-1-Ig targets NDRG2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Rat, Mouse, Pig samples.
| Tested Reactivity | Human, Rat, Mouse, Pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28612 Product name: Recombinant human NDRG2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-357 aa of BC010458 Sequence: MAELQEVQITEEKPLLPGQTPEAAKTHSVETPYVSVTFTVYGTPKPKRPAILTYHDVGLNYKSCFQPLFQFEDMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQYPSLDQLADMIPCVLQYLNFSTIIGVGVGAGAYILARYALNHPDTVEGLVLINIDPNAKGWMDWAAHKLTGLTSSIPEMILGHLFSQEELSGNSELIQKYRNIITHAPNLDNIELYWNSYNNRRDLNFERGGDITLRCPVMLVVGDQAPHEDAVVECNSKLDPTQTSFLKMADSGGQPQLTQPGKLTEAFKYFLQGMGYMASSCMTRLSRSRTASLTSAASVDGNRSRSRTLSQSSESGTLSSGPPGHTMEVSC Predict reactive species |
| Full Name | NDRG family member 2 |
| Calculated Molecular Weight | 371 aa, 39 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC010458 |
| Gene Symbol | NDRG2 |
| Gene ID (NCBI) | 57447 |
| RRID | AB_2882486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UN36 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDRG2 antibody 67191-1-Ig | Download protocol |
| WB protocol for NDRG2 antibody 67191-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NDRG2 antibody (Cat# 67191-1-Ig) has 4 citations published in Science Advances, Molecular Biomedicine, Scientific Reports, and Cancer Biotherapy & Radiopharmaceuticals. Research fields span neurovascular biology (blood-brain barrier disruption), hepatocellular carcinoma (lipid metabolism), reproductive biology (endometrial receptivity), and osteosarcoma (endoplasmic reticulum stress). Applications include IF, IHC, IP, and WB across human and mouse models.
| Species | Application | Title |
|---|---|---|
Sci Adv Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage | ||
Mol Biomed The tumor suppressor NDRG2 promotes ACC1 proteasomal degradation and inhibits de novo lipogenesis in hepatocellular carcinoma.
| ||
Sci Rep Exploring the mechanism of NDRG2 in regulating endometrial receptivity and embryo implantation. | ||
Cancer Biother Radiopharm Ultrasound-Enhanced Delivery of the Quercetin Derivative ANL3 Activates the FOXO1A-NDRG2-SOD2 Axis to Induce Endoplasmic Reticulum Stress in Osteosarcoma. |
Reviews
What customers say
The single review for this NDRG2 antibody gives it a 5-star rating. The reviewer used it for Western Blot on kidney tissue at a 1:1000 dilution and described it as the best NDRG2 antibody they have used for WB, praising the quality of the band it produced. Overall, the feedback is highly positive, highlighting strong performance in Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Uthra (Verified Customer) (10-02-2026) | Best antibody of NDRG2 for WB band.
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