NDUFA1 Polyclonal antibody

NDUFA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15561-1-AP

Synonyms

CI MWFE, Complex I MWFE, MWFE, NDUFA1, ZNF183

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Validation Data Gallery

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  WB analysis using 15561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15561-1-AP (NDUFA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 15561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15561-1-AP (NDUFA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue
• Positive IHC detected in: mouse liver tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15561-1-AP targets NDUFA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFA1 fusion protein Ag7206
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
• Calculated Molecular Weight: 7.5 kDa
• Observed Molecular Weight: 7.5 kDa
• GenBank Accession Number: BC000266
• Gene Symbol: NDUFA1
• Gene ID (NCBI): 4694
• RRID: AB_2918040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NDUFA1 gene encoding the MWFE polypeptide is located on the X chromosome. The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria, and it is predicted to be a membrane protein (PMID: 10200266).

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Changes and related factors of blood CCN1 levels in diabetic patients; Authors - Zhao-Yu Xiang
human	WB	Methods Mol Biol; Solvent-Induced Protein Precipitation for Drug Target Discovery; Authors - Xiaolei Zhang