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NDUFA10 Polyclonal antibody, PBS Only (32947-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 32947-1-AP (same clone as 32947-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.
WB analysis using 32947-1-AP (same clone as 32947-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 32947-1-AP (same clone as 32947-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 32947-1-AP (NDUFA10 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 32947-1-AP (same clone as 32947-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A431 cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 32947-1-AP (same clone as 32947-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

NDUFA10 Polyclonal antibody, PBS Only

Name: NDUFA10 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 32947-1-PBS

NDUFA10 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 32947-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CI-42kD, Complex I-42kD, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NADH:Ubiquinone Oxidoreductase Subunit A10, NADH-ubiquinone oxidoreductase 42 kDa subunit

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

32947-1-PBS targets NDUFA10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag37576 Product name: Recombinant human NDUFA10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-355 aa of BC003417 Sequence: MALRLLKLAATSASARVVAAGAQRVRGIHSSVQCKLRYGMWHFLLGDKASKRLTERSRVITVDGNICTGKGKLAKEIAEKLGFKHFPEAGIHYPDSTTGDGKPLATDYNGNCSLEKFYDDPRSNDGNSYRLQSWLYSSRLLQYSDALEHLLTTGQGVVLERSIFSDFVFLEAMYNQGFIRKQCVDHYNEVKSVTICDYLPPHLVIYIDVPVPEVQRRIQKKGDPHEMKITSAYLQDIENAYKKTFLPEMSEKCEVLQYSAREAQDSKKVVEDIEYLKFDKGPWLKQDNRTLYHLRLLVQDKFEVLNYTSIPIFLPEVTIGAHQTDRVLHQFRELPGRKYSPGYNTEVGDKWIWLK Predict reactive species
Full Name	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC003417
Gene Symbol	NDUFA10
Gene ID (NCBI)	4705
RRID	AB_3742801
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	O95299
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NDUFA10 (NADH:ubiquinone oxidoreductase subunit A10) is a subunit of mitochondrial complex I of the electron transport chain, encoding a protein with NADH dehydrogenase and oxidoreductase activity. This protein transfers electrons from NADH to the respiratory chain. Mutations in the NDUFA10 gene are associated with Leigh syndrome, a common mitochondrial energy metabolism disorder in children. Additionally, the expression levels of NDUFA10 are related to the occurrence and prognosis of various diseases. For example, in Alzheimer's disease, its low expression is associated with increased dementia severity. In prostate cancer, low expression of NDUFA10 predicts a poor prognosis.