CoraLite® Plus 488-conjugated NDUFA4L2 Monoclonal antibody

NDUFA4L2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1H10

Cat no : CL488-66050

Synonyms

FLJ26118, NDUFA4L2, NUOMS

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-66050

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL488-66050 (NDUFA4L2 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66050 is NDUFA4L2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFA4L2 fusion protein Ag9233
• Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
• Calculated Molecular Weight: 87 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC011910
• Gene Symbol: NDUFA4L2
• Gene ID (NCBI): 56901
• RRID: AB_2883240
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.