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NDUFA9 Recombinant monoclonal antibody, PBS Only (87143-2-PBS)

NDUFA9 Recombinant monoclonal antibody, PBS Only

Name: NDUFA9 Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87143-2-PBS-100UG

NDUFA9 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 87143-2-PBS

Clone No.252374E1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CI 39kD, CI-39kD, Complex I-39kD, NADH-ubiquinone oxidoreductase 39 kDa subunit, NDUFS2L

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Freight/Packing: -

Quantity

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Proteintech Guarantee

Filter: AllWBIHCIF/ICC

WB analysis using 87143-2-RR (same clone as 87143-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87143-2-RR (NDUFA9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of Human lung cancer （Adenocarcinoma） using 87143-2-RR (same clone as 87143-2-PBS)
Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87143-2-RR (NDUFA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse heart using 87143-2-RR (same clone as 87143-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87143-2-RR (NDUFA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 87143-2-RR (same clone as 87143-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA9 antibody (87143-2-RR, Clone: 252374E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87143-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87143-2-PBS targets NDUFA9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag16832 Product name: Recombinant human NDUFA9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-273 aa of BC009311 Sequence: MAAAAQSRVVRVLSMSRSAITAIATSVCHGPPCRQLHHALMPHGKGGRSSVSGIVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWDARDKDSIRRVVQHSNVVINLIGRDWETKNFDFEDVFVKIPQAIAQLSKEAGVEKFIHVSHLNANIKSSSRYLRNKAVGEKVVRDAFPEAIIVKPSDIFGREDRFLNSFASMHRFGPIPLGSLGWKTVKQPVYVVDVSKGIVNAVKDPDANGKSFAFVGPSRYLL Predict reactive species
Full Name	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa
Calculated Molecular Weight	377 aa, 43 kDa
Observed Molecular Weight	35 kDa
GenBank Accession Number	BC009311
Gene Symbol	NDUFA9
Gene ID (NCBI)	4704
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16795
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NDUFA9 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial) is also named as NDUFS2L, CI-39kD and belongs to the complex I NDUFA9 subunit family. It is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity.