NDUFB8 Polyclonal antibody

NDUFB8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14794-1-AP

Synonyms

ASHI, CI ASHI, Complex I ASHI, NDUFB8



Tested Applications

Positive WB detected inHepG2 cells, various samples, 3T3-L1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 11 publications below

Product Information

14794-1-AP targets NDUFB8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB8 fusion protein Ag6495
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight 19-22 kDa
GenBank accession numberBC000466
Gene symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFB8 antibody 14794-1-APDownload protocol
IHC protocol for NDUFB8 antibody 14794-1-APDownload protocol
IF protocol for NDUFB8 antibody 14794-1-APDownload protocol
IP protocol for NDUFB8 antibody 14794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Genet

AMPK-dependent and -independent coordination of mitochondrial function and muscle fiber type by FNIP1.

Authors - Liwei Xiao
humanWB

Aging (Albany NY)

Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism.

Authors - Delong Yin
mouseWB

Exp Gerontol

Optic atrophy 1 mediates coenzyme Q-responsive regulation of respiratory complex IV activity in brain mitochondria.

Authors - Kazuhide Takahashi
humanWB

J Biol Chem

Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.

Authors - Yanchun Ji
humanWB

Sci China Life Sci

Identification of mecciRNAs and their roles in the mitochondrial entry of proteins.

Authors - Xu Liu
mouseWB

Front Cell Dev Biol

Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.

Authors - Xiangyu Mao