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NDUFB8 Polyclonal antibody
NDUFB8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IP, IHC, IF, ELISA
Cat no : 14794-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, A549 cells, various samples, 3T3-L1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 27 publications below|
|IHC||See 1 publications below|
|IF||See 3 publications below|
14794-1-AP targets NDUFB8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NDUFB8 fusion protein Ag6495|
|Full Name||NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||19-22 kDa|
|GenBank accession number||BC000466|
|Gene ID (NCBI)||4714|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for NDUFB8 antibody 14794-1-AP||Download protocol|
|IHC protocol for NDUFB8 antibody 14794-1-AP||Download protocol|
|IF protocol for NDUFB8 antibody 14794-1-AP||Download protocol|
|IP protocol for NDUFB8 antibody 14794-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.
Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.
J Exp Med
FNIP1 regulates adipocyte browning and systemic glucose homeostasis in mice by shaping intracellular calcium dynamics.
Role of molybdenum in material immunomodulation and periodontal wound healing: Targeting immunometabolism and mitochondrial function for macrophage modulation.
J Cachexia Sarcopenia Muscle
MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism.
Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Edward (Verified Customer) (11-15-2022)
Used with 12 ug protein lysate NDUFB8 works well, both with ECL detection and fluorescent secondary antibodies it gives a strong band