|Positive WB detected in||A549 cells, K-562 cells, HeLa cells, HepG2 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue|
|Positive IHC detected in||human liver tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
67690-1-Ig targets NDUFB8 in WB, IHC, ELISA applications and shows reactivity with Human, mouse , rat, rabbit, pig samples.
|Tested Reactivity||Human, mouse , rat, rabbit, pig|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||NDUFB8 fusion protein Ag6569|
|Full Name||NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||18-22 kDa|
|GenBank accession number||BC000466|
|Gene ID (NCBI)||4714|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (06-17-2021)
Good and strong antibody, but with some non-specific bands