NDUFB8 Monoclonal antibody

NDUFB8 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat, rabbit, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B12C6

Cat no : 67690-1-Ig

Synonyms

ASHI, CI ASHI, Complex I ASHI, NDUFB8



Tested Applications

Positive WB detected inA549 cells, K-562 cells, HeLa cells, HepG2 cells, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67690-1-Ig targets NDUFB8 in WB, IHC, ELISA applications and shows reactivity with Human, mouse , rat, rabbit, pig samples.

Tested Reactivity Human, mouse , rat, rabbit, pig
Cited Reactivity mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NDUFB8 fusion protein Ag6569
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
Calculated molecular weight 22 kDa
Observed molecular weight18-22 kDa
GenBank accession numberBC000466
Gene symbol NDUFB8
Gene ID (NCBI) 4714
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFB8 antibody 67690-1-IgDownload protocol
IHC protocol for NDUFB8 antibody 67690-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Endocrinol (Lausanne)

Androgen Excess Induced Mitochondrial Abnormality in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome.

Authors - Linyi Song
mouseWB

Front Aging Neurosci

Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.

Authors - Keqiang He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (06-17-2021)

Good and strong antibody, but with some non-specific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: HepG2, mouse liver and mouse brain
NDUFB8 Antibody Western Blot validation (1:8000 dilution) in HepG2, mouse liver and mouse brain (Cat no:67690-1-Ig)