NDUFS5 Monoclonal antibody

NDUFS5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C2A5

Cat no : 66053-1-Ig

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 66053-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66053-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66053-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66053-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, HEK-293 cells, fetal human brain tissue
• Positive IHC detected in: human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66053-1-Ig is NDUFS5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NDUFS5 fusion protein Ag7663
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC001884
• Gene Symbol: NDUFS5
• Gene ID (NCBI): 4725
• RRID: AB_11043342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach’s NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.