NDUFS6 Polyclonal antibody

NDUFS6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14417-1-AP

Synonyms

NADH-ubiquinone oxidoreductase 13 kDa-A subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial, Complex I-13kD-A, Complex I 13kD A, CI-13kD-A

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Validation Data Gallery

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  WB analysis using 14417-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14417-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14417-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14417-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14417-1-AP (NDUFS6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14417-1-AP

  IP result of anti-NDUFS6 (IP:14417-1-AP, 4ug; Detection:14417-1-AP 1:300) with mouse liver tissue lysate 3200 ug.

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  IHC staining of human prostate cancer using 14417-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14417-1-AP (NDUFS6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 14417-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14417-1-AP (NDUFS6 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14417-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14417-1-AP (NDUFS6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, rat liver tissue, human liver tissue, human heart tissue, mouse heart tissue, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

14417-1-AP targets NDUFS6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFS6 fusion protein Ag5762
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 6, 13kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC046155
• Gene Symbol: NDUFS6
• Gene ID (NCBI): 4726
• RRID: AB_2149024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFS6(NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial) is also named as CI-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit and belongs to the complex I NDUFS6 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Publications

Species	Application	Title
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
human		Genes Dev; ALS/FTD-associated protein FUS induces mitochondrial dysfunction by preferentially sequestering respiratory chain complex mRNAs.; Authors - Yueh-Lin Tsai
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang
human	WB	Cancer Lett; PMID: 29906487; Authors - Linhua Lan
mouse	WB	FASEB J; PMID: 31530015; Authors - Rufeng Zhang
mouse	WB	Front Aging Neurosci; Hippocampus-Based Mitochondrial Respiratory Function Decline Is Responsible for Perioperative Neurocognitive Disorders.; Authors - Keqiang He