Biotin-conjugated NDUFS8 Recombinant monoclonal antibody

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• Biotin-conjugated NDUFS8 Recombinant monoclonal antibody (Biotin-87156)

NDUFS8 Uni-rAb^® Recombinant Antibody for IHC

Cat No. Biotin-87156

Clone No.252267D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2

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  IHC staining of mouse heart using Biotin-87156

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-87156 (NDUFS8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using Biotin-87156

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using Biotin-87156 (NDUFS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-87156 targets NDUFS8 in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18531

  Product name: Recombinant human NDUFS8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-210 aa of BC119754

  Sequence: MRCLTTPMLLRALAQAARAGPPGGRSLHSSAVAATYKYVNMQDPEMDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR

• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 210 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC119754
• Gene Symbol: NDUFS8
• Gene ID (NCBI): 4728
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00217
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NADH: ubiquinone oxidoreductase core subunit S8 (NDUFS8), also called TYKY, is an essential core subunit and component of the iron-sulfur (FeS) fragment of mitochondrial complex I directly involved in the electron transfer process and energy metabolism (PMID: 36557887). Pathogenic variants of the NDUFS8 are relevant to infantile-onset and severe diseases, including Leigh syndrome, cancer, and diabetes mellitus (PMID: 36557887). Knockdown of NDUFS8 impaired mitochondrial function, increased ROS, disrupted redox homeostasis, induced apoptosis, and suppressed proliferation and migration of HCC cells (PMID: 40914145).