NEDD4L Monoclonal antibody

NEDD4L Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2F6H6

Cat no : 67276-1-Ig

Synonyms

FLJ33870, hNedd4 2, KIAA0439, NEDD4 2, NEDD4.2, NEDD4L, NEDL3, RSP5



Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

67276-1-Ig targets NEDD4L in WB,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEDD4L fusion protein Ag4565
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Calculated molecular weight 854 aa, 96 kDa
Observed molecular weight 112 kDa, 125 kDa
GenBank accession numberBC032597
Gene symbol NEDD4L
Gene ID (NCBI) 23327
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Protocols

Product Specific Protocols
WB protocol for NEDD4L antibody 67276-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols