• Featured Product
  • KD/KO Validated

NEK1 Polyclonal antibody

NEK1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27146-1-AP

Synonyms

KIAA1901, NEK1, NimA related protein kinase 1, NY REN 55



Tested Applications

Positive WB detected inPC-12 cells, mouse pancreas tissue, HeLa cells
Positive IP detected inPC-12 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27146-1-AP targets NEK1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK1 fusion protein Ag25970
Full Name NIMA (never in mitosis gene a)-related kinase 1
Calculated Molecular Weight 1258 aa, 143 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC114491
Gene Symbol NEK1
Gene ID (NCBI) 4750
RRIDAB_2880773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NEK1 antibody 27146-1-APDownload protocol
IHC protocol for NEK1 antibody 27146-1-APDownload protocol
IF protocol for NEK1 antibody 27146-1-APDownload protocol
IP protocol for NEK1 antibody 27146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
humanWB,IHC

Cancers (Basel)

Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus

Authors - Lei Chen