NEK9 Polyclonal antibody

NEK9 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 51057-2-AP

Synonyms

KIAA1995, Nek8, NEK9, NERCC, NERCC1, Nercc1 kinase, NimA related kinase 8, NimA related protein kinase 9

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Validation Data Gallery

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  IF Staining of COS-7 using 51057-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed COS-7 cells using 51057-2-AP (NEK9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IF/ICC detected in: COS-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51057-2-AP targets NEK9 in IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEK9 fusion protein Ag1689
• Full Name: NIMA (never in mitosis gene a)- related kinase 9
• Calculated Molecular Weight: 107 kDa
• GenBank Accession Number: BC009336
• Gene Symbol: NEK9
• Gene ID (NCBI): 91754
• RRID: AB_2881241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is independently identified as a Nek6- and Ran GTPase- binding protein under a different name, Nercc1 (PMID:14660563). Nek9, together with the highly similar Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros (PMID: 23159858). The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer (PMID:21454704).