Product Information
51057-2-AP targets NEK9 in ELISA applications and shows reactivity with human, monkey samples.
| Tested Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1689 Product name: Recombinant human NEK9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2480-1/3 of BC009336 Sequence: PSGGFRGTMEADRGMEGLISPTEAMGNSNGASSSCPGWLRKELENAEFIPMPDSPSPLSAAFSESEKDTLPYEELQGLKVASEAPLEHKPQVEASSPRLNPAVTCAGKGTPLTPPACACSSLQVEVERLQGLVLKCLAEQQKLQQENLQIFTQLQKLNKKLEGGQQVGMHSKGTQTAKEEMEMDPKPDLDSDSWCLLGTDSCRPSL Predict reactive species |
| Full Name | NIMA (never in mitosis gene a)- related kinase 9 |
| Calculated Molecular Weight | 107 kDa |
| GenBank Accession Number | BC009336 |
| Gene Symbol | NEK9 |
| Gene ID (NCBI) | 91754 |
| RRID | AB_2881241 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD19 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is independently identified as a Nek6- and Ran GTPase- binding protein under a different name, Nercc1 (PMID:14660563). Nek9, together with the highly similar Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros (PMID: 23159858). The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer (PMID:21454704).
