|Positive WB detected in||BxPC-3 cells, HepG2 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||BxPC-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16418-1-AP targets NELFB in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NELFB fusion protein Ag9599|
|Full Name||cofactor of BRCA1|
|Calculated molecular weight||580aa,66 kDa; 475aa,54 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC011892|
|Gene ID (NCBI)||25920|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.
High constitutive activity of a broad panel of housekeeping and tissue-specific cis-regulatory elements depends on a subset of ETS proteins.
The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.