NELFB Polyclonal antibody

NELFB Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 16418-1-AP

Synonyms

COBRA1, cofactor of BRCA1, KIAA1182, Negative elongation factor B, NELF B, NELFB



Tested Applications

Positive WB detected inBxPC-3 cells, HepG2 cells
Positive IP detected inmouse brain tissue
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16418-1-AP targets NELFB in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NELFB fusion protein Ag9599
Full Name cofactor of BRCA1
Calculated molecular weight580aa,66 kDa; 475aa,54 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC011892
Gene symbol COBRA1
Gene ID (NCBI) 25920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NELFB antibody 16418-1-APDownload protocol
IF protocol for NELFB antibody 16418-1-APDownload protocol
IP protocol for NELFB antibody 16418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genes Dev

High constitutive activity of a broad panel of housekeeping and tissue-specific cis-regulatory elements depends on a subset of ETS proteins.

Authors - Alessia Curina
mouseIF

Nature

The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Authors - Bofeng Liu