Neudesin/NENF Monoclonal antibody

Neudesin/NENF Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4G9E12

Cat no : 60131-1-Ig

Synonyms

Neudesin, NENF, Protein GIG47, Neuron-derived neurotrophic factor, Cell immortalization-related protein 2

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Validation Data Gallery

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  WB analysis using 60131-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60131-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60131-1-Ig (NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

• 

  IHC staining of human colon using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 60131-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 60131-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1900 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HepG2 using 60131-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 60131-1-Ig(NENF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, HepG2 cells, LNCaP cells, A549 cells, HCT 116 cells, Jurkat cells, pig brain tissue
• Positive IHC detected in: human cerebellum tissue, human colon tissue, human colon cancer tissue, human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

60131-1-Ig targets Neudesin/NENF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Neudesin/NENF fusion protein Ag8387
• Full Name: neuron derived neurotrophic factor
• Calculated Molecular Weight: 172 aa, 19 kDa
• Observed Molecular Weight: 19 kDa, 16 kDa
• GenBank Accession Number: BC008823
• Gene Symbol: NENF
• Gene ID (NCBI): 29937
• RRID: AB_2150987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).

Publications

Species	Application	Title
human	WB	Cancer Cell Int; Identification of the tumor metastasis-related tumor subgroups overexpressed NENF in triple-negative breast cancer by single-cell transcriptomics; Authors - Guixin Wang