Tested Applications
| Positive WB detected in | Jurkat cells, Neuro-2a cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20246-1-AP targets Neogenin-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | neogenin homolog 1 (chicken) |
| Calculated Molecular Weight | 160 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | NM_002499 |
| Gene Symbol | Neogenin |
| Gene ID (NCBI) | 4756 |
| RRID | AB_10695051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92859 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEO1, also named as IGDCC2 and NGN, belongs to the immunoglobulin superfamily and DCC family. NEO1 may be involved as a regulatory protein in the transition of undifferentiated proliferating cells to their differentiated state. NEO1 may also function as a cell adhesion molecule in a broad spectrum of embryonic and adult tissues. This antibody is specific to NEO1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Neogenin-Specific antibody 20246-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Neogenin-Specific Polyclonal antibody (Cat# 20246-1-AP) has 6 total citations published in journals including J Adv Res, Cell Death Discov, Front Mol Neurosci, Hum Vaccin Immunother, Reproduction, and Sci Adv. Research fields span osteoporosis and osteogenesis, tumor progression and metastasis, visceral hypersensitivity and neuroscience, vaccine immunology, placental cell fusion, and neuronal cilia signaling. Applications cited include WB, IHC, and ELISA across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
J Adv Res UHRF1-mediated DNA 5-mC modification drives super-enhancer redistribution and impedes osteogenesis via TGM2-regulated autophagic flux in senile osteoporosis. | ||
Cell Death Discov Neogenin suppresses tumor progression and metastasis via inhibiting Merlin/YAP signaling | ||
Front Mol Neurosci Upregulation of Netrin-1 in the hippocampus mediates the formation of visceral hypersensitivity induced by maternal separation | ||
Hum Vaccin Immunother Hamster neogenin, a host-cell protein contained in a respiratory syncytial virus candidate vaccine, induces antibody responses in rabbits but not in clinical trial participants. | ||
Reproduction Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion. | ||
Sci Adv In situ proteomics unveils specialized domains for extrasynaptic signaling on neuronal cilia. |
