GNAS Monoclonal antibody
GNAS Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1A9C10
Cat no : 66253-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, pig brain tissue |
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells, human colon cancer tissue |
Positive FC detected in | MCF-7 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
66253-1-Ig targets GNAS in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Cited Reactivity | pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | GNAS fusion protein Ag20530 |
Full Name | GNAS complex locus |
Calculated molecular weight | 45 kDa |
Observed molecular weight | 46 kDa |
GenBank accession number | BC002722 |
Gene symbol | GNAS |
Gene ID (NCBI) | 2778 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GNAS antibody 66253-1-Ig | Download protocol |
IHC protocol for GNAS antibody 66253-1-Ig | Download protocol |
IF protocol for GNAS antibody 66253-1-Ig | Download protocol |
FC protocol for GNAS antibody 66253-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Vet Microbiol Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein |