GNAS Monoclonal antibody

GNAS Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A9C10

Cat no : 66253-1-Ig

Synonyms

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH, Protein ALEX

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Validation Data Gallery

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  WB analysis of fetal human brain using 66253-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66253-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 66253-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 66253-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 66253-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human colon cancer using 66253-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human colon cancer using 66253-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human colon cancer using 66253-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of MCF-7 using 66253-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human GNAS (66253-1-Ig, Clone:1A9C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 66253-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human GNAS (66253-1-Ig, Clone:1A9C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, pig brain tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, human colon cancer tissue
• Positive FC detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

66253-1-Ig targets GNAS in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNAS fusion protein Ag20530
• Full Name: GNAS complex locus
• Calculated molecular weight: 45 kDa
• Observed molecular weight: 46 kDa
• GenBank accession number: BC002722
• Gene symbol: GNAS
• Gene ID (NCBI): 2778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Publications

Species	Application	Title
pig	WB	Vet Microbiol; Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein; Authors - Jiaping Zhu