GNAS Monoclonal antibody

GNAS Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1A9C10

Cat no : 66253-1-Ig

Synonyms

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH, Protein ALEX



Tested Applications

Positive WB detected infetal human brain tissue, pig brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66253-1-Ig targets GNAS in WB, IHC,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAS fusion protein Ag20530
Full Name GNAS complex locus
Calculated molecular weight 45 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002722
Gene symbol GNAS
Gene ID (NCBI) 2778
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Protocols

Product Specific Protocols
WB protocol for GNAS antibody 66253-1-IgDownload protocol
IHC protocol for GNAS antibody 66253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols