CoraLite® Plus 488-conjugated GNAS Monoclonal antibody

GNAS Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A9C10

Cat no : CL488-66253

Synonyms

AHO, C20orf45, dJ309F20.1.1, dJ806M20.3.3, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, NESP55, PHP1A, PHP1B, POH, Protein ALEX



Tested Applications

Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66253 targets GNAS in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAS fusion protein Ag20530
Full Name GNAS complex locus
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002722
Gene Symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_2919305
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GNAS antibody CL488-66253Download protocol
FC protocol for CL Plus 488 GNAS antibody CL488-66253Download protocol
Standard Protocols
Click here to view our Standard Protocols