Tested Applications
| Positive WB detected in | Y79 cells |
| Positive IHC detected in | human stomach tissue, mouse stomach tissue, mouse heart tissue, rat heart tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12740-1-AP targets NET1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3636 Product name: Recombinant human NET1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 142-543 aa of BC010285 Sequence: ARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV Predict reactive species |
| Full Name | neuroepithelial cell transforming 1 |
| Calculated Molecular Weight | 596 aa, 68 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC010285 |
| Gene Symbol | NET1 |
| Gene ID (NCBI) | 10276 |
| RRID | AB_2923558 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z628 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NET1 antibody 12740-1-AP | Download protocol |
| IHC protocol for NET1 antibody 12740-1-AP | Download protocol |
| WB protocol for NET1 antibody 12740-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NET1 Polyclonal antibody (Cat# 12740-1-AP) has 2 total citations. These appear in Oncology Reports and Human Pathology. The research fields include bladder cancer, where miR-22-3p targeting NET1 enhances multi-chemoresistance, and cervical squamous cell carcinoma, where siRNA-mediated NET1 knockdown reduces angiogenesis and tumor growth via VEGF down-regulation. Applications cited include Western Blot and IHC.
| Species | Application | Title |
|---|---|---|
Oncol Rep miR‑22‑3p enhances multi‑chemoresistance by targeting NET1 in bladder cancer cells. | ||
Hum Pathol Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation. |
