NEUROD1 Polyclonal antibody, PBS Only

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• NEUROD1 Polyclonal antibody, PBS Only (12081-1-PBS)

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  WB analysis using 12081-1-AP (same clone as 12081-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12081-1-AP (NEUROD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

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  IP experiment of Y79 using 12081-1-AP (same clone as 12081-1-PBS)

  IP result of anti-NEUROD1 (IP:12081-1-AP, 3ug; Detection:12081-1-AP 1:200) with Y79 cells lysate 2000ug. This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12081-1-AP (same clone as 12081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12081-1-AP (same clone as 12081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12081-1-AP (same clone as 12081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 12081-1-AP (same clone as 12081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12081-1-AP (same clone as 12081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12081-1-AP (same clone as 12081-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 12081-1-AP (same clone as 12081-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 12081-1-AP (same clone as 12081-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12081-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NEUROD1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 12081-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

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Product Information

12081-1-PBS targets NEUROD1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2713

  Product name: Recombinant human NEUROD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-356 aa of BC009046

  Sequence: MTKSYSESGLMGEPQPQGPPSWTDECLSSQDEEHEADKKEDDLEAMNAEEDSLRNGGEEEDEDEDLEEEEEEEEEDDDQKPKRRGPKKKKMTKARLERFKLRRMKANARERNRMHGLNAALDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKSPDLVSFVQTLCKGLSQPTTNLVAGCLQLNPRTFLPEQNQDMPPHLPTASASFPVHPYSYQSPGLPSPPYGTMDSSHVFHVKPPPHAYSAALEPFFESPLTDCTSPSFDGPLSPPLSINGNFSFKHEPSAEFEKNYAFTMHYPAATLAGAQSHGSIFSGTAAPRCEIPIDNIMSFDSHSHHERVMSAQLNAIFHD

• Full Name: neurogenic differentiation 1
• Calculated Molecular Weight: 356 aa, 40 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC009046
• Gene Symbol: NEUROD1
• Gene ID (NCBI): 4760
• RRID: AB_2877823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13562
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.