NF-M Monoclonal antibody, PBS Only

NF-M Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E3B12

Cat no : 66396-1-PBS

Synonyms

160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

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Validation Data Gallery

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  WB analysis of rat brain using 66396-1-Ig (same clone as 66396-1-PBS)

  Lysates of rat brain, mouse brain tissues and PC-12 cells were subjected to SDS PAGE followed by western blot with 66396-1-Ig (NEFM Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66396-1-Ig (same clone as 66396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66396-1-Ig (same clone as 66396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66396-1-Ig (same clone as 66396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NF-M antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66396-1-Ig (same clone as 66396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NEFM antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66396-1-Ig (same clone as 66396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66396-1-Ig (NEFM antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 66396-1-Ig (same clone as 66396-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M (66396-1-Ig, Clone:2E3B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66396-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66396-1-PBS targets NF-M in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NF-M fusion protein Ag22709
• Full Name: neurofilament, medium polypeptide
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC002421
• Gene Symbol: NEFM
• Gene ID (NCBI): 4741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.