NF-M Monoclonal antibody

NF-M Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat







Cat no : 66396-1-Ig


160 kDa neurofilament protein, NEF3, NEFM, neurofilament, Neurofilament 3, NF, NF M, NFM, NF-M

Tested Applications

Positive WB detected inrat brain tissue, rat brain, mouse brain tissue, PC-12 cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-12 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66396-1-Ig targets NF-M in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-M fusion protein Ag22709
Full Name neurofilament, medium polypeptide
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC002421
Gene Symbol NEFM
Gene ID (NCBI) 4741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,140-160 kDa and NF-H, 200-220 kDa.


Product Specific Protocols
WB protocol for NF-M antibody 66396-1-IgDownload protocol
IHC protocol for NF-M antibody 66396-1-IgDownload protocol
FC protocol for NF-M antibody 66396-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols