Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human meningioma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21686-1-AP targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16265 Product name: Recombinant human NF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 245-595 aa of BC020257 Sequence: DPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL Predict reactive species |
| Full Name | neurofibromin 2 (merlin) |
| Calculated Molecular Weight | 595 aa, 70 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC020257 |
| Gene Symbol | NF2 |
| Gene ID (NCBI) | 4771 |
| RRID | AB_10792410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35240 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
| IHC protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
| IP protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
| WB protocol for Neurofibromin 2 antibody 21686-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-NF2 (Merlin) antibody (Cat# 21686-1-AP) has 15 total citations. Publications appear in journals including Science Advances, EMBO Molecular Medicine, FASEB Journal, Journal of Translational Medicine, BMC Cancer, Molecular Neurobiology, Journal of Cellular and Molecular Medicine, Archives of Pathology & Laboratory Medicine, Thyroid, Microorganisms, Journal of Cellular Physiology, Cancer Management Research, Biochemical and Biophysical Research Communications, and OncoTargets and Therapy. Research fields span oncology (liver, glioblastoma, mesothelioma, thyroid, bladder, breast, colon, renal, osteosarcoma, lung cancers), ferroptosis, Hippo/YAP signaling, cell cycle regulation, and diabetic nephropathy.
| Species | Application | Title |
|---|---|---|
Sci Adv Cyclin K condensates bridge CDK12 to phosphorylate and drive oncogenic YAP activation in hepatocellular carcinoma. | ||
J Transl Med PXD101 inhibits malignant progression and radioresistance of glioblastoma by upregulating GADD45A | ||
EMBO Mol Med De novo pyrimidine synthesis is a collateral metabolic vulnerability in NF2-deficient mesothelioma
| ||
Thyroid MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer. | ||
Mol Neurobiol Taurine Attenuates Neuronal Ferroptosis by CSF-Derived Exosomes of GABABR Encephalitis Through GABABR/NF2/P-YAP Pathway | ||
J Cell Mol Med DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis |
Reviews
What customers say
Both reviewers used this antibody for Western Blot and reported positive results. One user tested it at 1:2000 dilution on Jurkat E6 cells and rated it 4 out of 5, noting it "worked well." The other tested it at 1:1000 dilution on mouse and human pancreatic cancer cell lines, giving it a perfect 5 out of 5 with the comment "Worked well." Overall, users found the antibody reliable and effective for WB applications across different cell types and dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Titouan (Verified Customer) (11-12-2025) | worked well
 |
Bettina (Verified Customer) (08-04-2022) | Worked well
 |
