Validation Data Gallery
|Positive WB detected in
|HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue
|Positive IP detected in
|Positive IHC detected in
|mouse brain tissue, human meningioma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:750-1:3000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
21686-1-AP targets NF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
|human, mouse, rat, canine
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|NF2 fusion protein Ag16265
|neurofibromin 2 (merlin)
|Calculated molecular weight
|595 aa, 70 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
|Product Specific Protocols
|WB protocol for NF2 antibody 21686-1-AP
|IHC protocol for NF2 antibody 21686-1-AP
|IF protocol for NF2 antibody 21686-1-AP
|IP protocol for NF2 antibody 21686-1-AP
|Click here to view our Standard Protocols
MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.
J Cell Mol Med
Onco Targets Ther
PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.
Cancer Manag Res
MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.
Biochem Biophys Res Commun
A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.
J Cell Physiol
Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.
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Bettina (Verified Customer) (08-04-2022)