NF2 Polyclonal antibody

NF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21686-1-AP

Synonyms

ACN, BANF, Merlin, Neurofibromin 2, neurofibromin 2 (merlin), NF2, SCH, Schwannomerlin, Schwannomin



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman meningioma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21686-1-AP is NF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF2 fusion protein Ag16265
Full Name neurofibromin 2 (merlin)
Calculated molecular weight 595 aa, 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC020257
Gene symbol NF2
Gene ID (NCBI) 4771
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NF2 antibody 21686-1-APDownload protocol
IHC protocol for NF2 antibody 21686-1-APDownload protocol
IF protocol for NF2 antibody 21686-1-APDownload protocol
IP protocol for NF2 antibody 21686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Physiol

Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.

Authors - Yan-Jing Zhang
humanWB

J Cell Mol Med

PMID: 32281270

Authors - Jie Liu
human,mouseWB

Thyroid

MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.

Authors - Chunxiao Yu
humanWB

Biochem Biophys Res Commun

A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.

Authors - Yuen Gao
humanWB

Cancer Manag Res

MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.

Authors - Hua-Chun Rao
humanIF

Onco Targets Ther

PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.

Authors - Lai Wei
  • KD Validated