NF2 Polyclonal antibody

NF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 21686-1-AP

Synonyms

ACN, BANF, Merlin, Neurofibromin 2, neurofibromin 2 (merlin), NF2, SCH, Schwannomerlin, Schwannomin

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Validation Data Gallery

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  WB analysis of HeLa using 21686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21686-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21686-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21686-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21686-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 21686-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 21686-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21686-1-AP

  IP Result of anti-NF2 (IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:600) with HeLa cells lysate 2400ug.

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  IHC staining of human meningioma using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human meningioma using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 21686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21686-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21686-1-AP (NF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human meningioma tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21686-1-AP is NF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF2 fusion protein Ag16265
• Full Name: neurofibromin 2 (merlin)
• Calculated molecular weight: 595 aa, 70 kDa
• Observed molecular weight: 66-70 kDa
• GenBank accession number: BC020257
• Gene symbol: NF2
• Gene ID (NCBI): 4771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Onco Targets Ther; PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.; Authors - Lai Wei ; KD Validated
human	WB	Cancer Manag Res; MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.; Authors - Hua-Chun Rao
human	WB	Biochem Biophys Res Commun; A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.; Authors - Yuen Gao
human,mouse	WB	Thyroid; MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.; Authors - Chunxiao Yu
human	WB	J Cell Mol Med; PMID: 32281270; Authors - Jie Liu
rat	WB	J Cell Physiol; Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.; Authors - Yan-Jing Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bettina (Verified Customer) (08-04-2022)

Worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse & human pancreatic cancer cell lines

NF2 Antibody Western Blot validation (1:1000 dilution) in mouse & human pancreatic cancer cell lines (Cat no:21686-1-AP)