NF2 Polyclonal antibody

NF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21686-1-AP

Synonyms

ACN, BANF, Merlin, Neurofibromin 2, neurofibromin 2 (merlin), NF2, SCH, Schwannomerlin, Schwannomin



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman meningioma tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21686-1-AP is NF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF2 fusion protein Ag16265
Full Name neurofibromin 2 (merlin)
Calculated molecular weight 595 aa, 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC020257
Gene symbol NF2
Gene ID (NCBI) 4771
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NF2 antibody 21686-1-APDownload protocol
IHC protocol for NF2 antibody 21686-1-APDownload protocol
IF protocol for NF2 antibody 21686-1-APDownload protocol
IP protocol for NF2 antibody 21686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Onco Targets Ther

PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.

Authors - Lai Wei
  • KD Validated
humanWB

Cancer Manag Res

MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.

Authors - Hua-Chun Rao
humanWB

Biochem Biophys Res Commun

A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.

Authors - Yuen Gao
human,mouseWB

Thyroid

MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.

Authors - Chunxiao Yu
humanWB

J Cell Mol Med

PMID: 32281270

Authors - Jie Liu
ratWB

J Cell Physiol

Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.

Authors - Yan-Jing Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bettina (Verified Customer) (08-04-2022)

Worked well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse & human pancreatic cancer cell lines
NF2 Antibody Western Blot validation (1:1000 dilution) in mouse & human pancreatic cancer cell lines (Cat no:21686-1-AP)