Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 21686-1-AP (same clone as 21686-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
WB analysis using 21686-1-AP (same clone as 21686-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
WB analysis of SH-SY5Y using 21686-1-AP (same clone as 21686-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 21686-1-AP (same clone as 21686-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 21686-1-AP (same clone as 21686-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 21686-1-AP (same clone as 21686-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 21686-1-AP (same clone as 21686-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 21686-1-AP (same clone as 21686-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 21686-1-AP (same clone as 21686-1-PBS)
IP result of anti-NF2 (IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IHC staining of human meningioma using 21686-1-AP (same clone as 21686-1-PBS)
Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 21686-1-AP (same clone as 21686-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NF2 antibody (21686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 21686-1-AP (same clone as 21686-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21686-1-AP (NF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21686-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Neurofibromin 2 Polyclonal antibody, PBS Only

Name: Neurofibromin 2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21686-1-PBS

Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21686-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21686-1-PBS targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16265 Product name: Recombinant human NF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 245-595 aa of BC020257 Sequence: DPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL Predict reactive species
Full Name	neurofibromin 2 (merlin)
Calculated Molecular Weight	595 aa, 70 kDa
Observed Molecular Weight	66-70 kDa
GenBank Accession Number	BC020257
Gene Symbol	NF2
Gene ID (NCBI)	4771
RRID	AB_10792410
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35240
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.