NFAT5 Polyclonal antibody, PBS Only

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• NFAT5 Polyclonal antibody, PBS Only (21713-1-PBS)

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  WB analysis using 21713-1-AP (same clone as 21713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21713-1-AP (same clone as 21713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21713-1-AP (same clone as 21713-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFAT5 antibody (21713-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21713-1-PBS in a different storage buffer formulation.

NFAT5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21713-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

21713-1-PBS targets NFAT5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16341

  Product name: Recombinant human NFAT5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1231-1531 aa of BC146765

  Sequence: MFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF

• Full Name: nuclear factor of activated T-cells 5, tonicity-responsive
• Calculated Molecular Weight: 1531 aa, 166 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC146765
• Gene Symbol: NFAT5
• Gene ID (NCBI): 10725
• RRID: AB_3085667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94916
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).