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NFE2L1 Polyclonal antibody, PBS Only (12936-1-PBS)

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WB analysis of mouse skeletal muscle using 12936-1-AP (same clone as 12936-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 12936-1-AP (same clone as 12936-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 12936-1-AP (same clone as 12936-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 12936-1-AP (same clone as 12936-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12936-1-AP (same clone as 12936-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 12936-1-AP (same clone as 12936-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 12936-1-AP (same clone as 12936-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 12936-1-AP (same clone as 12936-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 12936-1-AP (same clone as 12936-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12936-1-AP (same clone as 12936-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 12936-1-AP (same clone as 12936-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of Fetal mouse bone using 12936-1-AP (same clone as 12936-1-PBS)
Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
WB analysis of Fetal mouse bone using 12936-1-AP (same clone as 12936-1-PBS)
Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
IP experiment of mouse skeletal muscle using 12936-1-AP (same clone as 12936-1-PBS)
IP result of anti-NFE2L1 (IP:12936-1-AP, 4ug; Detection:12936-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug. This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 12936-1-AP (same clone as 12936-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12936-1-AP (same clone as 12936-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12936-1-AP (same clone as 12936-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 12936-1-AP (same clone as 12936-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12936-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NFE2L1 Polyclonal antibody, PBS Only

Name: NFE2L1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12936-1-PBS

NFE2L1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12936-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12936-1-PBS targets NFE2L1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3916 Product name: Recombinant human NFE2L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 293-742 aa of BC010623 Sequence: MQAMEVNTSASEILYSAPPGDPLSTNYSLAPNTPINQNVSLHQASLGGCSQDFLLFSPEVESLPVASSSTLLPLAPSNSTSLNSTFGSTNLTGLFFPPQLNGTANDTAGPELPDPLGGLLDEAMLDEISLMDLAIEEGFNPVQASQLEEEFDSDSGLSLDSSHSPSSLSSSEGSSSSSSSSSSSSSSASSSASSSFSEEGAVGYSSDSETLDLEEAEGAVGYQPEYSKFCRMSYQDPAQLSCLPYLEHVGHNHTYNMAPSALDSADLPPPSALKKGSKEKQADFLDKQMSRDEHRARAMKIPFTNDKIINLPVEEFNELLSKYQLSEAQLSLIRDIRRRGKNKMAAQNCRKRKLDTILNLERDVEDLQRDKARLLREKVEFLRSLRQMKQKVQSLYQEVFGRLRDENGRPYSPSQYALQYAGDGSVLLIPRTMADQQARRQERKPKDRRK Predict reactive species
Full Name	nuclear factor (erythroid-derived 2)-like 1
Calculated Molecular Weight	772 aa, 85 kDa
Observed Molecular Weight	65-120 kDa
GenBank Accession Number	BC010623
Gene Symbol	NFE2L1
Gene ID (NCBI)	4779
RRID	AB_2267298
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14494
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580).