Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, pig liver tissue, HL-60 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, PC-12 cells, L02 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach tissue, human kidney tissue, human prostate cancer tissue, mouse kidney tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10268-1-AP targets IkB Alpha in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, bovine, ducks |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0345 Product name: Recombinant human NFKBIA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-237 aa of BC002601 Sequence: GLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLL Predict reactive species |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC002601 |
| Gene Symbol | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_2151423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| IF protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| IHC protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| IP protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| WB protocol for IkB Alpha antibody 10268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IκB Alpha polyclonal antibody (Cat# 10268-1-AP) has 881 total citations published in journals including Nature Communications, Nature Metabolism, Nature Cell Biology, Cell Metabolism, Science Advances, EMBO Journal, Molecular Cell, Autophagy, Cell Death & Disease, Theranostics, Advanced Science, Phytomedicine, and Biomaterials. Associated research fields encompass NF-κB signaling, inflammation, oncology, neuroinflammation, immunology, autophagy, gut microbiota, liver disease, wound healing, cardiology, and metabolic disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells | ||
J Hematol Oncol Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer. | ||
Mol Cancer Activation of the NF-¿B pathway as a mechanism of alcohol enhanced progression and metastasis of human hepatocellular carcinoma. | ||
Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Nat Metab MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1α promotes tumour immune evasion. |
Reviews
What customers say
Customers report strong performance for this antibody in Western Blot and Immunohistochemistry. Two reviewers gave 5-star ratings, noting clean results with no nonspecific bands in cancer cells and reliable staining in Caco-2 cells and animal colon tissue at a 1:1000 dilution. One reviewer gave 4 stars, mentioning weak bands in mouse heart lysates at basal state. Overall, the antibody is well-received for its specificity and sensitivity, with minor variability in signal strength depending on tissue type and expression levels.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
P (Verified Customer) (07-15-2025) | Used for caco2 cells and animal colon tissue
|
SONAM (Verified Customer) (10-04-2023) | Antibody worked well. No nonspecific band was observed.
|
CHAO (Verified Customer) (01-30-2020) | Weak bands for the heart lysates at basal state.
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