Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, HL-60 cells, A431 cells, pig liver tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, NIH/T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, mouse kidney tissue, human stomach tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10268-1-AP targets IkB Alpha in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|Tested Reactivity||human, mouse, pig, rat|
|Cited Reactivity||human, mouse, rat, CHICKEN, ducks, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IkB Alpha fusion protein Ag0345|
|Full Name||nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC002601|
|Gene ID (NCBI)||4792|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
|Product Specific Protocols|
|WB protocol for IkB Alpha antibody 10268-1-AP||Download protocol|
|IHC protocol for IkB Alpha antibody 10268-1-AP||Download protocol|
|IF protocol for IkB Alpha antibody 10268-1-AP||Download protocol|
|IP protocol for IkB Alpha antibody 10268-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
CHAO (Verified Customer) (01-30-2020)
Weak bands for the heart lysates at basal state.