IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat and More (3)

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Publications

421

Reviews (2)

Cat no : 10268-1-AP

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

---

Validation Data Gallery

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 10268-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10268-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10268-1-AP

  IP result of anti-IkB Alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:15000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 10268-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10268-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10268-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IkB Alpha (10268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HEK-293 cells, pig liver tissue, HL-60 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, PC-12 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue, mouse kidney tissue, human prostate cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells, HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 403 publications below
• IHC: See 12 publications below
• IF: See 6 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

10268-1-AP targets IkB Alpha in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Cited Reactivity: human, mouse, rat, CHICKEN, ducks, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IkB Alpha fusion protein Ag0345
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002601
• Gene Symbol: NFKBIA
• Gene ID (NCBI): 4792
• RRID: AB_2151423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB,IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
mouse	WB	Sci Adv; Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.; Authors - Yuanjiu Lei
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

SONAM (Verified Customer) (10-04-2023)

Antibody worked well. No nonspecific band was observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells

FH

CHAO (Verified Customer) (01-30-2020)

Weak bands for the heart lysates at basal state.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C

NFKB1,p105,p50 Polyclonal antibody

14220-1-AP

IKBKB Polyclonal antibody

15649-1-AP

IkB Beta Polyclonal antibody

12660-1-AP