IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 51066-1-AP

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA



Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

51066-1-AP targets IkB Alpha in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC002601
Gene symbol NFKBIA
Gene ID (NCBI) 4792
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Protocols

Product Specific Protocols
WB protocol for IkB Alpha antibody 51066-1-APDownload protocol
IHC protocol for IkB Alpha antibody 51066-1-APDownload protocol
IP protocol for IkB Alpha antibody 51066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biochim Biophys Acta

Minimally modified LDL upregulates endothelin type B receptors in rat coronary artery via ERK1/2 MAPK and NF-κB pathways.

Authors - Jie Li L
humanWB

Med Sci Monit

PMID: 29332931

Authors - Yunjing Zhang
mouseWB

Life Sci

TL1A primed dendritic cells activation exacerbated chronic murine colitis.

Authors - Fei Han
mouseWB

Nutrients

Fish Hydrolysate Supplementation Containing n-3 Long Chain Polyunsaturated Fatty Acids and Peptides Prevents LPS-Induced Neuroinflammation.

Authors - Mathilde Chataigner
mouseWB

Front Immunol

Serine Supports IL-1β Production in Macrophages Through mTOR Signaling.

Authors - Siyuan Chen
humanWB

J Immunol

Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation.

Authors - Zheng Zhenhua Z