NFS1 Monoclonal antibody, PBS Only

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  WB analysis of HSC-T6 using 67021-1-Ig (same clone as 67021-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67021-1-Ig (same clone as 67021-1-PBS)

  WB result of NFS1 antibody (67021-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFS1 transfected HepG2 cells. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67021-1-Ig (same clone as 67021-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 67021-1-Ig (same clone as 67021-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67021-1-Ig (same clone as 67021-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67021-1-Ig (same clone as 67021-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67021-1-Ig (same clone as 67021-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67021-1-Ig (NFS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67021-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NFS1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67021-1-PBS

Clone No.3A10A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

3A10A7, Cysteine desulfurase, EC:2.8.1.7, HUSSY 08, HUSSY-08

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Product Information

67021-1-PBS targets NFS1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8017

  Product name: Recombinant human NFS1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-189 aa of BC018471

  Sequence: MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKTESHSVIQAGVQWHDLDSLQAPPPGFTPFSCLSLLSSWDYRRPPPHPANFLYF

• Full Name: NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC018471
• Gene Symbol: NFS1
• Gene ID (NCBI): 9054
• RRID: AB_2918480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y697
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.