Tested Applications
| Positive WB detected in | HSC-T6 cells, HepG2 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67021-1-Ig targets NFS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8017 Product name: Recombinant human NFS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-189 aa of BC018471 Sequence: MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKTESHSVIQAGVQWHDLDSLQAPPPGFTPFSCLSLLSSWDYRRPPPHPANFLYF Predict reactive species |
| Full Name | NFS1 nitrogen fixation 1 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC018471 |
| Gene Symbol | NFS1 |
| Gene ID (NCBI) | 9054 |
| RRID | AB_2918480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y697 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NFS1 antibody 67021-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NFS1 Monoclonal antibody (3A10A7), catalog number 67021-1-Ig, has 4 total citations. These appear in Redox Biology, Advanced Science, and Free Radical Biology and Medicine. The associated research fields include liver cancer metastasis and ferroptosis resistance, abdominal aortic aneurysm regulation, viral replication and lipid metabolism, and oxidative stress-induced matrix degradation with ferroptosis and senescence in nucleus pulposus cells.
| Species | Application | Title |
|---|---|---|
Redox Biol Histone lactylation drives liver cancer metastasis by facilitating NSF1-mediated ferroptosis resistance after microwave ablation
| ||
Adv Sci (Weinh) Ferritinophagy Rewires Carnitine-Dependent Lipid Metabolism to Inhibit PRRSV and IAV Replication. | ||
Adv Sci (Weinh) NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2.
| ||
Free Radic Biol Med GLS1-mediated glutamine metabolism mitigates oxidative stress-induced matrix degradation, ferroptosis, and senescence in nucleus pulposus cells by modulating Fe2+ homeostasis |
