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NFS1 Monoclonal antibody

NFS1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3A10A7

Cat no : 67021-1-Ig

Synonyms

HUSSY 08, IscS, NFS1, NIFS



Tested Applications

Positive WB detected inHSC-T6 cells, HepG2 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67021-1-Ig targets NFS1 in WB,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFS1 fusion protein Ag8017
Full Name NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Calculated molecular weight 50 kDa
GenBank accession numberBC018471
Gene symbol NFS1
Gene ID (NCBI) 9054
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for NFS1 antibody 67021-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols