XLF Monoclonal antibody

XLF Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b









Cat no : 66552-1-Ig


NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-30000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66552-1-Ig targets XLF in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XLF fusion protein Ag2496
Full Name nonhomologous end-joining factor 1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC030986
Gene Symbol XLF
Gene ID (NCBI) 79840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.


Product Specific Protocols
WB protocol for XLF antibody 66552-1-IgDownload protocol
IHC protocol for XLF antibody 66552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols