NHEJ1 Monoclonal antibody

NHEJ1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F8C7

Cat no : 66552-1-Ig

Synonyms

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-30000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66552-1-Ig targets NHEJ1 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NHEJ1 fusion protein Ag2496
Full Name nonhomologous end-joining factor 1
Calculated molecular weight 35 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC030986
Gene symbol NHEJ1
Gene ID (NCBI) 79840
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule. 

Protocols

Product Specific Protocols
WB protocol for NHEJ1 antibody 66552-1-IgDownload protocol
IHC protocol for NHEJ1 antibody 66552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols