XLF Monoclonal antibody
XLF Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
WB, IHC, ELISA
Cat no : 66552-1-Ig
|Positive WB detected in
|HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
|Positive IHC detected in
|human testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:5000-30000
|IHC : 1:500-1:2000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66552-1-Ig targets XLF in WB, IHC, ELISA applications and shows reactivity with Human samples.
|Host / Isotype
|Mouse / IgG2b
|XLF fusion protein Ag2496
|nonhomologous end-joining factor 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.