NHLRC1 Polyclonal antibody

NHLRC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21310-1-AP

Synonyms

EPM2B, MALIN, NHL repeat containing 1, NHLRC1

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Validation Data Gallery

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  WB analysis of L02 using 21310-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21310-1-AP (NHLRC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21310-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21310-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 21310-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21310-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 21310-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21310-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21310-1-AP (NHLRC1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human heart tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21310-1-AP is NHLRC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NHLRC1 fusion protein Ag15940
• Full Name: NHL repeat containing 1
• Calculated Molecular Weight: 395 aa, 42 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC103888
• Gene Symbol: NHLRC1
• Gene ID (NCBI): 378884
• RRID: AB_10733882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHLRC1(NHL repeat-containing protein 1) is also named as Malin,EPM2B. It is predicted to encode a 42.3-kDa (395amino acid) protein, malin, containing a detectable zinc-binding RING finger and six NHL-repeat domains(PMID:12958597).It can suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS).Defects in NHLRC1 are a cause of progressive myoclonic epilepsy type 2 (EPM2).This antibody is specific to NHLRC1.