NHP2 Recombinant monoclonal antibody

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• NHP2 Recombinant monoclonal antibody (86677-1-RR)

• 

  WB analysis using 86677-1-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 86677-1-RR (NHP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86677-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NHP2 antibody (86677-1-RR, Clone: 251700B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

NHP2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86677-1-RR

Clone No.251700B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

HSPC286, NHP 2, NHP2P, NOLA2

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Tested Applications

• Positive WB detected in: Caco-2 cells, Jurkat cells, HeLa cells, SH-SY5Y cells, K-562 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86677-1-RR targets NHP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7251

  Product name: Recombinant human NHP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-153 aa of BC000009

  Sequence: MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMVLAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVKPHEEYQEAYDECLEEVQSLPLPL

• Full Name: NHP2 ribonucleoprotein homolog (yeast)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17-22 kDa
• GenBank Accession Number: BC000009
• Gene Symbol: NHP2
• Gene ID (NCBI): 55651
• RRID: AB_3745048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NX24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.