NHP2L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NHP2L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15802-1-AP

Synonyms

SNU13, OTK27, NHP2-like protein 1, N-terminally processed, NHP2-like protein 1, hSNU13

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Validation Data Gallery

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  WB analysis using 15802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15802-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15802-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 15802-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15802-1-AP (NHP2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 15802-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15802-1-AP (NHP2L1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 15802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NHP2L1 antibody (15802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, human brain tissue, mouse pancreas tissue, HepG2 cells, Jurkat cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human cervical cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

15802-1-AP targets NHP2L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NHP2L1 fusion protein Ag8532
• Full Name: NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 128 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC005358
• Gene Symbol: NHP2L1
• Gene ID (NCBI): 4809
• RRID: AB_2251452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHP2L1, belongs to the ribosomal protein L7Ae family, is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs, and is required for subsequent recruitment of PRP31 and activation of the spliceosomal complex [PMID: 17412961]. It binds to the 5'-stem-loop of U4 snRNA and may have a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding [PMID: 10545122].

Publications

Species	Application	Title
human	WB	Mol Cell; Nascent Pre-rRNA Sorting via Phase Separation Drives the Assembly of Dense Fibrillar Components in the Human Nucleolus.; Authors - Run-Wen Yao ; KO Validated
human	IF	Mol Cell; Condensates induced by transcription inhibition localize active chromatin to nucleoli.; Authors - Takaaki Yasuhara
human	WB	Mol Cell; LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.; Authors - Xin Wang
mouse	WB	Biochim Biophys Acta Proteins Proteom; Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.; Authors - Uchenna Emechebe