Ninein Polyclonal antibody

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• Ninein Polyclonal antibody (13007-1-AP)

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• KD/KO Validated

Ninein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13007-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NIN, KIAA1565, hNinein, GSK3B-interacting protein, GSK3B interacting protein

• 

  WB analysis using 13007-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13007-1-AP (Ninein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 13007-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 13007-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13007-1-AP targets Ninein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3688

  Product name: Recombinant human Ninein protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1870-2020 aa of BC034708

  Sequence: VRQLESNLLPKHQKHLNPSGTMNPTEQEKLSLKRECDQFQKEQSPANRKVSQMNSLEQELETIHLENEGLKKKQVKLDEQLMEMQHLRSTATPSPSPHAWDLQLLQQQACPMVPREQFLQLQRQLLQAERINQHLQEELENRTSETNTPQ

• Full Name: ninein (GSK3B interacting protein)
• Calculated Molecular Weight: 2090 aa, 243 kDa
• Observed Molecular Weight: 240-255 kDa
• GenBank Accession Number: BC034708
• Gene Symbol: Ninein
• Gene ID (NCBI): 51199
• RRID: AB_3085405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N4C6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

Publications

What published studies show

The Ninein Polyclonal antibody (catalog #13007-1-AP) has 6 citations. These appear in Nature Communications (×2), Developmental Cell, EMBO Journal, Developmental Dynamics, and Genes Disease. The research spans cilia and centriole biology, including basal foot architecture, planar polarity in multiciliated cells, centriole engagement, central pair assembly, ciliary transition zone condensates, and a TRIOBP variant linked to Meniere disease. Applications cited include immunofluorescence and Western blot in human and mouse samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Nat Commun; DEUP1 functions as a scaffold for basal foot integrity and planar polarity in multiciliated cells.; Authors - Haeryung Lee
human	WB,IF	Dev Cell; Comparative Super-Resolution Mapping of Basal Feet Reveals a Modular but Distinct Architecture in Primary and Motile Cilia.; Authors - Quynh P H Nguyen ; KD Validated
human	IF	EMBO J; Multimodal mechanisms of human centriole engagement and disengagement; Authors - Kei K Ito
mouse	IF	Dev Dyn; Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.; Authors - Nozawa Yoko Inès YI
human	IF	Nat Commun; CFAP100 forms phase-separated condensates required for ciliary transition zone assembly.; Authors - Shuang Sun
		Genes Dis; A rare variant in TRIOBP linked to occupational noise exposure in Meniere disease.; Authors - Pablo Cruz-Granados