Anti-Mouse NK1.1 (CD161) (PK136)

NK1.1 (CD161) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

PK136

Cat no : 65138-1-Ig

Synonyms

CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a

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Validation Data Gallery

• 

  FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD49b (DX5) and 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

• 

  FC experiment of C57BL/6 mouse splenocytes using 65138-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse NK1.1 (CD161) (65138-1-Ig, Clone:PK136) and FITC anti-Mouse IgG2a Antibody at dilution 1:100, or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) and FITC anti-Mouse IgG2a Antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive FC detected in: C57BL/6 mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65138-1-Ig targets NK1.1 (CD161) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: NK-1+ cells from mouse spleen and bone marrow
• Full Name: killer cell lectin-like receptor subfamily B member 1A
• GenBank Accession Number: BC120707
• Gene Symbol: Klrb1a
• Gene ID (NCBI): 17057
• RRID: AB_2918425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

NK1.1 (CD161), also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).